Annexin A10 Antibody from MyBioSource.com

Annexins (Anx) are a structurally related family of calcium /phospholipid-binding and actin regulatory proteins comprise of AnxA1-A10. These proteins are found in both intracellular and extra cellular space. These are involved in the regulation of a wide range of molecular and cellular processes mediated by Ca-signaling. The name annexin, is given since their main property is to bind (i.e., to annex). The basic structure of an annexin is composed of two major domains namely the core (C-terminus) and the head regions (N-terminal). The core region binds calcium and phospholipids at the plasma membrane. The N terminal region is important for providing a binding site for cytoplasmic proteins. Phosphorylation in some Annexins causes affinity changes for calcium in the core region or alters the protein interaction at the cytoplasmic side.

AnxA10, a novel member of the Annexin family, found to be down-regulated in cancer cell lines and it's over expression has led to regression of cell growth and increased in cells associated with apoptotic cells. This shows that Annexin 10 acts as a tumor suppressor gene. It has been recently considered as a potential gene therapy target for hepatocellular carcinoma due to its reduced expression associated with vascular invasion, early recurrence, and poor prognosis associated with the p53 mutation (1). AnxA10 has several distinct features, including rare expression, codon deletion in conserved repeat 3, and an unusual ablation of the type II calcium-binding sites in tetrad core repeats (2). The loss of type II calcium-binding sites is a fundamental deviation from Annexin structure conservation and undoubtedly has functional consequences for membrane association and aggregation properties of annexin proteins (2). AnxA10 is mapped to chromosome 4q33, a regions commonly associated with a high frequency of allelic loss in hepatocellular carcinoma (2).

AnxA10- selective antibodies were generated against a peptide taken form within the N-region spanning 60-105aa. The AnxA10-selective antibodies are affinity purified on an immobilized antigen based affinity matrix, the isolated antibodies were then stabilized in antibody stabilization buffer for long-term storage. The AnxA10-selective antibodies are fully characterized for applications in western blotting and ELISA at the recommended dilutions